Product overview

Specific primers and probes are designed against the CHD gene on the avian sex chromosomes; after fluorescent PCR amplification the sex is determined from the product pattern. Suitable for sex identification in parrots, pigeons and many other bird species, and a reliable solution when the sex of a young bird cannot be told from its appearance.

Technical features

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High specificity

Specific primers and probes are designed against conserved pathogen regions to reduce non-specific amplification.

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High sensitivity

Fluorescent probe chemistry is sensitive enough to detect low-copy-number nucleic acid.

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Anti-contamination

A built-in dUTP/UNG system removes false positives caused by residual PCR aerosol.

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Reliable results

Supplied with internal-control quality checks to support confidence in the process and the result.

Workflow

1 Sample collection: collect throat swabs, cloacal swabs or faecal samples using standard technique
2 Nucleic acid extraction: extract DNA/RNA from the sample
3 Set-up and amplification: add the reaction mix and run the amplification programme
4 Result interpretation: read the result from the fluorescence signal together with the internal control

Bird Sex Identification Fluorescent PCR Kit