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Pigeon Paramyxovirus Type 1 (PPMV-1) RT-PCR Kit

Pigeon paramyxovirus (Newcastle disease)

Pathogen Detection

Product overview

An RT-PCR fluorescent assay designed for pigeon paramyxovirus type 1 (PPMV-1, the pigeon Newcastle disease virus). Newcastle disease is one of the most important notifiable diseases in racing pigeons; this kit detects viral nucleic acid to support clinical diagnosis and disease monitoring.

Technical features

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High specificity

Specific primers and probes are designed against conserved pathogen regions to reduce non-specific amplification.

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High sensitivity

Fluorescent probe chemistry is sensitive enough to detect low-copy-number nucleic acid.

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Anti-contamination

A built-in dUTP/UNG system removes false positives caused by residual PCR aerosol.

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Reliable results

Supplied with internal-control quality checks to support confidence in the process and the result.

Workflow

  1. 1 Sample collection: collect throat swabs, cloacal swabs or faecal samples using standard technique
  2. 2 Nucleic acid extraction: extract DNA/RNA from the sample
  3. 3 Set-up and amplification: add the reaction mix and run the amplification programme
  4. 4 Result interpretation: read the result from the fluorescence signal together with the internal control

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